Platelet-rich fibrin induces the differentiation of bone marrow mesenchymal stem cells into
Schwann cells

doi:10.3969/j.issn.2095-4344.2012.49.007

Abstract

Abstract:

BACKGROUND: Platelet-rich fibrin is known as a novel platelet condensate. Several studies have demonstrated that platelet-rich fibrin can induce the differentiation of bone marrow mesenchymal stem cells into neuronal-like cells.
OBJECTIVE: To investigate the effects of platelet-rich fibrin on differentiation of bone marrow mesenchymal stem cells into Schwann cells.
METHODS: Rabbit bone marrow mesenchymal stem cells were isolated and randomized to three groups. In the control group, conventional antioxidants were used. In the platelet-rich fibrin 1 group, one piece of platelet-rich fibrin was added to induce cell differentiation. In the platelet-rich fibrin 2 group, two pieces of platelet-rich fibrin were added to induce cell differentiation.
RESULTS AND CONCLUSION: At days 3, 7, 14, 21 days of culture, CCK-8 assay was performed to detect cell toxicity. Results showed that cell proliferation at each time point was faster in the platelet-rich fibrin 1 group than that in the control group (P < 0.05), and it was faster in the platelet-rich fibrin 2 group than that in the platelet-rich fibrin 1 group (P < 0.05). Real-time PCR results showed that at day 21 of culture, intracellular S-100 and glial fibrillary acidic protein mRNA expression levels were significantly higher in the platelet-rich fibrin 1 group than those that in the control group (P < 0.05), and no significant differences were observed between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05). Flow cytometry results showed that at day 21 of culture, the proportions of intracellular S-100 and glial fibrillary acidic protein positive cells in the platelet-rich fibrin 1 group were significantly higher than those in the control group (P > 0.05) and there was no significant difference between platelet-rich fibrin 1 group and platelet-rich fibrin 2 group (P > 0.05). Results indicate that platelet-rich fibrin can promote the proliferation of bone marrow mesenchymal stem cells in a dose-dependent manner, and platelet-rich fibrin can induce bone marrow mesenchymal stem cells to differentiate into a high proportion of Schwann cells. The platelet-rich fibrin induction is superior to conventional chemical inducation.

CLC Number:

R394.2

Feng Yu-hua, Dong Jing, Lu Lei, Li Qi, Song Lei. Platelet-rich fibrin induces the differentiation of bone marrow mesenchymal stem cells into
Schwann cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9157-9161.

Figures/Tables 3

References

[1] Choukroun J, Adda F, Schoeffler C, et al. Une opportunite’ en paro-implantologie: le PRF. Implantodontie. 2000; 42: 55-62.

[2] Dohan DM, Choukroun J, Diss A,et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e45-50.

[3] Lundquist R, Dziegiel MH, Agren MS. Bioactivity and stability of endogenous fibrogenic factors in platelet-rich fibrin. Wound Repair Regen. 2008;16(3):356-363.

[4] Farrag TY, Lehar M, Verhaegen P,et al. Effect of platelet rich plasma and fibrin sealant on facial nerve regeneration in a rat model. Laryngoscope. 2007;117(1):157-165.

[5] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30. 中华人民共和国科学技术部. 关于善待实验动物的指导性意见.2006-09-30.

[6] Guo XM, Wang CY, Wang YH,et al. Experimental study of the isolation, culture and in chondrogenic differentiation of human bone mesenchymal stem cell.Zhonghua Kou Qiang Yi Xue Za Zhi. 2003;38(1):63-66.

[7] Romero-Ramirez L, Cao H, Regalado MP,et al. X box-binding protein 1 regulates angiogenesis in human pancreatic adenocarcinomas.Transl Oncol. 2009;2(1):31-38.

[8] Dohan DM, Choukroun J, Diss A,et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3):e37-44.

[9] Kikuchi M, Yatabe M, Tando Y,et al. Immunohistochemical localization of anterior pituitary hormones in S-100 protein-positive cells in the rat pituitary gland. Cell Tissue Res. 2011;345(3):425-429.

[10] Eng LF, Ghirnikar RS, Lee YL. Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000). Neurochem Res. 2000; 25(9-10):1439-1451.

[11] Mantur M, Koper O. Platelet- derived growth factor--the construction, role and it's receptors. Pol Merkur Lekarski. 2008;24(140):173-176.

[12] Movaghar B, Tiraihi T, Mesbah-Namin SA.Transdifferentiation of bone marrow stromal cells into Schwann cell phenotype using progesterone as inducer. Brain Res. 2008;1208:17-24.

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